烟台毓璜顶医院

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鉴定专家

刘芙君

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刘芙君,男,博士,副主任技师,硕士生导师。
毕业于北京大学获博士学位,青岛大学附属烟台毓璜顶医院中心实验室主任,山东省干细胞工程技术研究中心主任,烟台毓璜顶医院DNA遗传实验室负责人,烟台毓璜顶医院法医司法鉴定所所长、授权签字人、技术负责人、国家司法鉴定人,青岛大学附属烟台毓璜顶医院医学实验学教研室主任,烟台市生命科学技术研究中心主任,国家自然科学基金通信评审专家,主持完成山东省自然科学基金项目、国家自然科学基金项目等科研课题,在国内外期刊杂志上发表论文60余篇,获评山东省自然科学二等奖、山东医学科技创新成果奖二等奖、烟台市自然科学奖二等奖。社会兼职:中国医药生物技术协会科研实验室建设与管理分会全国常务委员,中国医药生物技术协会组织生物样本库分会全国委员,山东省医师协会组织样本库及转化医学专业委员会副主任委员,中国细胞生物学学会干细胞生物学分会会员,烟台市医学会医学实验室建设与管理专业委员会主任委员潍坊医学院、青岛大学硕士研究生导师。

 

    

法医遗传标记,法医STR分型,亲子鉴定,司法亲权鉴定,亲缘关系鉴定等。

 

学历背景

2015.09 2019.07,北京大学,免疫学,博士

2004.09 2007.07,青岛大学,遗传学,硕士

1997.09 2000.07,滨州医学院,医学实验技术,大学

 

学术论文

1.Liu X, Wang Z, Liu F. Chronic exposure of BPA impairs male germ cell proliferation and induces lower sperm quality in male mice. Chemosphere. 2021 Jan;262:127880. doi: 10.1016/j.chemosphere.2020.127880. Epub 2020 Aug 5. PubMed PMID: 32777607.
2.Liu X, Wang Y, Zhu P, Wang J, Liu J, Li N, Wang W, Zhang W, Zhang C, Wang Y, Shen X, Liu F. Human follicular fluid proteome reveals association between overweight status and oocyte maturation abnormality. Clin Proteomics. 2020;17:22. doi: 10.1186/s12014-020-09286-7. eCollection 2020. PubMed PMID: 32528235; PubMed Central PMCID: PMC7282111.
3.Liu F, Liu X, Liu X, Li T, Zhu P, Liu Z, Xue H, Wang W, Yang X, Liu J, Han W. Integrated Analyses of Phenotype and Quantitative Proteome of CMTM4 Deficient Mice Reveal Its Association with Male Fertility. Mol Cell Proteomics. 2019 Jun;18(6):1070-1084. doi: 10.1074/mcp.RA119.001416. Epub 2019 Mar 13. PubMed PMID: 30867229; PubMed Central PMCID: PMC6553932.
4.Liu X, Li Q, Wang W, Liu F. Aberrant expression of sperm‑specific glycolytic enzymes are associated with poor sperm quality. Mol Med Rep. 2019 Apr;19(4):2471-2478. doi: 10.3892/mmr.2019.9926. Epub 2019 Feb 1. PubMed PMID: 30720094; PubMed Central PMCID: PMC6423621.
5.Liu X, Wang X, Liu F. Decreased expression of heat shock protein A4L in spermatozoa is positively related to poor human sperm quality. Mol Reprod Dev. 2019 Apr;86(4):379-386. doi: 10.1002/mrd.23113. Epub 2019 Jan 22. PubMed PMID: 30637842.
6.Wang WJ, Zhang H, Chen ZQ, Zhang W, Liu XM, Fang JY, Liu FJ, Kwak-Kim J. Endometrial TGF-
β, IL-10, IL-17 and autophagy are dysregulated in women with recurrent implantation failure with chronic endometritis. Reprod Biol Endocrinol. 2019 Jan 3;17(1):2. doi: 10.1186/s12958-018-0444-9. PubMed PMID: 30606202; PubMed Central PMCID: PMC6317248.
7.Liu XX, Cai L, Liu FJ. An in silico analysis of human sperm genes associated with asthenozoospermia and its implication in male infertility. Medicine (Baltimore). 2018 Dec;97(49):e13338. doi: 10.1097/MD.0000000000013338. PubMed PMID: 30544396; PubMed Central PMCID: PMC6310515.
8.Liu X, Wang W, Zhu P, Wang J, Wang Y, Wang X, Liu J, Li N, Wang X, Lin C, Liu F. In-depth quantitative proteome analysis of seminal plasma from men with oligoasthenozoospermia and normozoospermia. Reprod Biomed Online. 2018 Oct;37(4):467-479. doi: 10.1016/j.rbmo.2018.06.025. Epub 2018 Jul 19. PubMed PMID: 30396457.
9.Liu X, Liu G, Liu J, Zhu P, Wang J, Wang Y, Wang W, Li N, Wang X, Zhang C, Shen X, Liu F. iTRAQ-based analysis of sperm proteome from normozoospermic men achieving the rescue-ICSI pregnancy after the IVF failure. Clin Proteomics. 2018;15:27. doi: 10.1186/s12014-018-9203-3. eCollection 2018. PubMed PMID: 30166971; PubMed Central PMCID: PMC6102933.
10.Shen X, Liu X, Zhu P, Zhang Y, Wang J, Wang Y, Wang W, Liu J, Li N, Liu F. Proteomic analysis of human follicular fluid associated with successful in vitro fertilization. Reprod Biol Endocrinol. 2017 Jul 27;15(1):58. doi: 10.1186/s12958-017-0277-y. PubMed PMID: 28750635; PubMed Central PMCID: PMC5530927.
11.Wang WJ, Liu FJ, Zhang X, Liu XM, Qu QL, Li FH, Zhuang LL, Li XX, Hao CF. Periodic elevation of regulatory T cells on the day of embryo transfer is associated with better in vitro fertilization outcome. J Reprod Immunol. 2017 Feb;119:49-53. doi: 10.1016/j.jri.2017.01.002. Epub 2017 Jan 19. PubMed PMID: 28122284.
12.Liu XX, Shen XF, Liu FJ. Screening targeted testis‑specific genes for molecular assessment of aberrant sperm quality. Mol Med Rep. 2016 Aug;14(2):1594-600. doi: 10.3892/mmr.2016.5434. Epub 2016 Jun 23. PubMed PMID: 27356588; PubMed Central PMCID: PMC4940090.
13.Wang X, Liu F, Gao X, Liu X, Kong X, Wang H, Li J. Comparative proteomic analysis of heat stress proteins associated with rat sperm maturation. Mol Med Rep. 2016 Apr;13(4):3547-52. doi: 10.3892/mmr.2016.4958. Epub 2016 Mar 2. PubMed PMID: 26936680.
14.Liu X, Jin SH, Liu XX, Wang WJ, Liu FJ. Proteome profiling of the sperm maturation milieu in the rhesus monkey (Macaca mulatta) epididymis. Reprod Fertil Dev. 2016 Apr;28(6):732-41. doi: 10.1071/RD14322. PubMed PMID: 25354248.
15.Liu XX, Zhang H, Shen XF, Liu FJ, Liu J, Wang WJ. Characteristics of testis-specific phosphoglycerate kinase 2 and its association with human sperm quality. Hum Reprod. 2016 Feb;31(2):273-9. doi: 10.1093/humrep/dev301. Epub 2015 Dec 17. PubMed PMID: 26677959.
16.Wang K, Li N, Yeung CH, Cooper TG, Liu XX, Liu J, Wang WT, Li Y, Shi H, Liu FJ. Comparison of gene expression of the oncogenic Wnt/
β-catenin signaling pathway components in the mouse and human epididymis. Asian J Androl. 2015 Nov-Dec;17(6):1006-11. doi: 10.4103/1008-682X.157396. PubMed PMID: 26228040; PubMed Central PMCID: PMC4814947.
17.Liu X, Liu FJ, Jin SH, Shen XF, Wang YW. In-depth Proteomic mapping of mouse (Mus musculus) epididymal constructive basis for sperm maturation. Proteome Sci. 2015;13:20. doi: 10.1186/s12953-015-0076-3. eCollection 2015. PubMed PMID: 26225126; PubMed Central PMCID: PMC4518611.
18.Liu X, Liu F. In‑depth mapping of human testicular and epididymal proteins and their functional association with spermatozoa. Mol Med Rep. 2015 Jul;12(1):173-9. doi: 10.3892/mmr.2015.3435. Epub 2015 Mar 5. PubMed PMID: 25760095; PubMed Central PMCID: PMC4438928.
19.Liu X, Liu FJ, Jin SH, Wang YW, Liu XX, Zhu P, Wang WT, Liu J, Wang WJ. Comparative proteome analysis of human testis from newborn, young adult, and aged men identified spermatogenesis-associated proteins. Electrophoresis. 2015 Jun 1;. doi: 10.1002/elps.201500135. [Epub ahead of print] PubMed PMID: 26031402.
20.Liu XX, Liu FJ. Novel bioinformatic identification of differentially expressed tissue-specific and cancer-related proteins from the Human Protein Atlas for biomarker discovery. Genet Mol Res. 2015 May 4;14(2):4557-65. doi: 10.4238/2015.May.4.14. PubMed PMID: 25966229.
21.Liu FJ, Liu X, Han JL, Wang YW, Jin SH, Liu XX, Liu J, Wang WT, Wang WJ. Aged men share the sperm protein PATE1 defect with young asthenozoospermia patients. Hum Reprod. 2015 Apr;30(4):861-9. doi: 10.1093/humrep/dev003. Epub 2015 Jan 29. PubMed PMID: 25637620.
22.Liu X, Wang W, Liu F. New insight into the castrated mouse epididymis based on comparative proteomics. Reprod Fertil Dev. 2015 Mar;27(3):551-6. doi: 10.1071/RD13323. PubMed PMID: 24513102.
23.Hua XF, Wang YW, Tang YX, Yu SQ, Jin SH, Meng XM, Li HF, Liu FJ, Sun Q, Wang HY, Li JY. Pancreatic insulin-producing cells differentiated from human embryonic stem cells correct hyperglycemia in SCID/NOD mice, an animal model of diabetes. PLoS One. 2014;9(7):e102198. doi: 10.1371/journal.pone.0102198. eCollection 2014. PubMed PMID: 25009980; PubMed Central PMCID: PMC4092102.
24.Liu FJ, Wang XB, Cao AG. Screening and functional analysis of a differential protein profile of human breast cancer. Oncol Lett. 2014 Jun;7(6):1851-1856. doi: 10.3892/ol.2014.1978. Epub 2014 Mar 18. PubMed PMID: 24932247; PubMed Central PMCID: PMC4049688.
25.Wang WJ, Liu FJ, Xin-Liu, Hao CF, Bao HC, Qu QL, Liu XM. Adoptive transfer of pregnancy-induced CD4+CD25+ regulatory T cells reverses the increase in abortion rate caused by interleukin 17 in the CBA/JxBALB/c mouse model. Hum Reprod. 2014 May;29(5):946-52. doi: 10.1093/humrep/deu014. Epub 2014 Feb 20. PubMed PMID: 24556316.
26.Hua XF, Wang XB, Liu FJ. Functional analysis of human cancer-associated genes and their association with the testes and epididymis. Oncol Lett. 2013 Sep;6(3):811-816. doi: 10.3892/ol.2013.1450. Epub 2013 Jul 8. PubMed PMID: 24137416; PubMed Central PMCID: PMC3789015.
27.Wang WJ, Liu FJ, Qu HM, Hao CF, Qu QL, Xiong-Wang, Bao HC, Wang XR. Regulation of the expression of Th17 cells and regulatory T cells by IL-27 in patients with unexplained early recurrent miscarriage. J Reprod Immunol. 2013 Sep;99(1-2):39-45. doi: 10.1016/j.jri.2013.04.002. Epub 2013 May 31. PubMed PMID: 23731956.
28.Liu FJ, Hua XF, Wang WJ. A new bioinformatics insight into human cancer-associated proteins. Oncol Rep. 2012 Jun;27(6):1932-6. doi: 10.3892/or.2012.1714. Epub 2012 Mar 12. PubMed PMID: 22426468.
29.Li Y, Wang HY, Wan FC, Liu FJ, Liu J, Zhang N, Jin SH, Li JY. Deep sequencing analysis of small non-coding RNAs reveals the diversity of microRNAs and piRNAs in the human epididymis. Gene. 2012 Apr 15;497(2):330-5. doi: 10.1016/j.gene.2012.01.038. Epub 2012 Jan 31. PubMed PMID: 22313525.
30.Wang Y, Xu C, Wang H, Liu J, Hui S, Li N, Liu F, Li J. Efficient derivation of human embryonic stem cell lines from discarded embryos through increases in the concentration of basic fibroblast growth factor. Hum Cell. 2012 Mar;25(1):16-23. doi: 10.1007/s13577-011-0039-7. PubMed PMID: 22287285.
31.Liu F, Wang H, Li J. An integrated bioinformatics analysis of mouse testis protein profiles with new understanding. BMB Rep. 2011 May;44(5):347-51. doi: 10.5483/BMBRep.2011.44.5.347. PubMed PMID: 21615991.
32.Li J, Liu F, Liu X, Liu J, Zhu P, Wan F, Jin S, Wang W, Li N, Liu J, Wang H. Mapping of the human testicular proteome and its relationship with that of the epididymis and spermatozoa. Mol Cell Proteomics. 2011 Mar;10(3):M110.004630. doi: 10.1074/mcp.M110.004630. Epub 2010 Dec 22. PubMed PMID: 21178120; PubMed Central PMCID: PMC3047158.
33.Liu F, Jin S, Li N, Liu X, Wang H, Li J. Comparative and functional analysis of testis-specific genes. Biol Pharm Bull. 2011;34(1):28-35. doi: 10.1248/bpb.34.28. PubMed PMID: 21212513.
34.Li J, Liu F, Wang H, Liu X, Liu J, Li N, Wan F, Wang W, Zhang C, Jin S, Liu J, Zhu P, Liu Y. Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins. Mol Cell Proteomics. 2010 Nov;9(11):2517-28. doi: 10.1074/mcp.M110.001719. Epub 2010 Aug 24. PubMed PMID: 20736409; PubMed Central PMCID: PMC2984238.

 

承担项目

1.国家自然科学基金面上项目,81971438,睾丸特异性蛋白CMTM2参与精子发生、精子质量调控的功能及机制研究,2020.01-2013.1255万元,在研,主持
2.国家自然科学基金青年项目,81300533PATE-B和α7 nAChR相互作用促进人精子运动的功能研究,2014.01-2016.12, 23, 已结题,主持
3.国家自然科学基金面上项目,81370013,男性附睾精子成熟微环境蛋白深度解析及其临床意义研究,2016.01-2019.1255万元,已结题,参加(第二位)
4.国家自然科学基金面上项目,81370013Th17/Treg免疫在原因不明复发性流产发病机制中的作用之深入研究,2014.01-2017.1270万元,已结题,参加(第二位)
5.国家自然科学基金应急管理项目,81741027,调节性T细胞自噬异常在原因不明复发性流产发病机制中的作用,2018.01-2018.1220万元,已结题,参加(第二位)
6.山东省自然科学基金面上项目,ZR2019MH035,睾丸特异表达基因Cmtm2b敲除致雄性不育的功能研究,2019.7-2022.6,20万,在研,主持
7.山东省自然科学基金青年项目,ZR2013HQ002,人类附睾高表达PATE蛋白调节CHRNA7活性促进精子顶体反应的机制研究,2013.1-2015.126万元,已结题,主持
8.山东省自然科学基金面上项目,2014ZRA06009,男性精子成熟的微环境蛋白质分子基础深入研究,2014.01-2016.1211万元,已结题,参加(第二位)

 

荣誉获奖

2014,人类附睾精子成熟整体功能基因组学研究,山东省自然科学奖二等奖,3/5
2014Th17/Treg 免疫在原因不明复发性流产发病机制中的作用, 烟台市自然科学奖三等奖,3/5
2013,体外定向诱导人胚胎干细胞分化为胰岛细胞移植研究, 烟台市自然科学奖二等奖,3/7
2015,体外定向诱导人胚胎干细胞分化为胰岛细胞移植研究,山东省医学科技进步三等奖,3/7
2017PGK2在人精子质量评价中的应用,烟台市科技进步三等奖,2/7

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